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Why rt pcr test is done – why rt pcr test is done:.RT PCR Test – What It Is & How It Is Done

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Why rt pcr test is done – why rt pcr test is done:.Rt Pcr vs Pcr

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According to microbiologists, RT-PCR revolutionized the way clinical microbiology laboratories diagnose many human microbial infection. According to microbiologists it revolutionized the way clinical microbiology laboratories diagnose many human microbial infection.

According to the American Society for Microbiology, the combination of excellent sensitivity and specificity, low contamination risk, and speed has made real-time PCR technology an appealing alternative to culture- or immunoassay-based testing methods for diagnosing many infectious diseases?

It is an RNA virus, that means it infiltrates a healthy cell to multiply and survive. This sample is treated with several chemical solutions that remove substances, such as proteins and fats, and extracts only the RNA present in the sample. As per an international document standard real-time RT-PCR setup usually goes through 35 cycles, which means that by the end of the process, around 35 billion new copies of the sections of viral DNA are created from each strand of the virus present in the sample.

As new copies of the viral DNA sections are built, the marker labels attach to the DNA strands and then release a fluorescent dye, which is measured by the machine’s computer and presented in real-time on the screen. The computer tracks the amount of fluorescence in the sample after each cycle.

When the amount goes over a certain level of fluorescence, this confirms that the virus is present. Scientists also monitor how many cycles it takes to reach this level in order to estimate the severity of the infection: the fewer the cycles, the more severe the viral infection is. The state itself had incidents of false positives and false negatives.

But it does not rule out bacterial infection or co-infection with other viruses. The agent detected may not be the definite cause of disease. Also, negative results do not preclude the infection and should not be used as the sole basis for patient management decisions. Negative results must be combined with clinical observations, patient history, and epidemiological information. Only trained clinical laboratory personnel specifically instructed and trained in the techniques of real-time PCR and in vitro diagnostic procedures could carry out the RT-PCR Tests.

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Why rt pcr test is done – why rt pcr test is done: –

 
The causative agent for Covid19 is the SARS-CoV-2 virus. It is an RNA virus, that means it infiltrates a healthy cell to multiply and survive. Certain viruses, including COVID, are made up of RNA rather than DNA. For these viruses, the RNA must be changed into DNA before copying. This process is called reverse transcription PCR (rtPCR). PCR and rtPCR. RT-PCR tests are very accurate when properly performed by a health care professional, but the rapid test can miss some cases. Antigen test.

 

– COVID diagnostic testing – Mayo Clinic

 

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